Coding

Part:BBa_K3868042

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-01)

P17

Promoter of trans-2-enoyl-CoA reductase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 658
    Illegal PstI site found at 735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 783
    Illegal XhoI site found at 48
    Illegal XhoI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 735
    Illegal AgeI site found at 756
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 90


[edit]
Categories
Parameters
None